Detail of Grant Awarded Academic Researches
Survey of Tick Habitat Distribution in the Tama River Basin and Survey of Tick-borne Zoonoses
(at that time)
|Laboratory of Veterinary Public Health, Department of Veterinary Medical Sciences,Graduate of School of Agricultural and Life Sciences, The University of Tokyo Associate Professor Professor|
In October 2015 and from March to October 2016, we collected tick samples on footpaths along the Tama River. As a result, we collected a total of 551 samples (21 Haemaphysalis megaspinosa, 68 H. flava, 102 H. longicornis, 1 H. inermis Birula, 2 Ixodes ovatus, and 357 Haemaphysalis larvae). From these, we prepared 266 specimens, with each specimen consisting of one imago, one nymph, and between one and five larvae. After grinding these ticks with beads and extracting their RNA, we detected the genes of various pathogens by means of the RT-PCR technique. The results were all negative for severe fever with thrombocytopenia syndrome virus (SFTSV) and Flavivirus genes. There were positive results for Rickettsia 17-kD gene in 2/266 and gltA gene in 6/266, with a high detection sensitivity for the gltA gene. In addition, there was a positive result for the Flavivirus L-segment gene in 1/266. In addition to 17-kD amplified using the PCR technique, the gltA gene and the Flavivirus L-segment gene, we analyzed the S-segment gene. The sequence of the Rickettsia gltA gene was identical in all six specimens, with a 98.8% match to R. japonica and a 100% match to Candidatus R. Vini and R. spp. Hf332. Analysis of the Flavivirus revealed it to be the first to be reported in Japan of a strain in the same cluster as Yongjia tick virus and Lesvos virus.
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