Detail of Grant Awarded Academic Researches
Project |
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Growth characteristic, reproductive characteristic and conservation measures of endangered species (CR+EN), Pyropia tenera (Bangiales, Rhodophyta). |
Academic Research |
No.306 |
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Principal Investigator |
Satoshi Shimada |
Affiliation | Graduate School of Humanities and Sciences, Ochanomizu University |
Research Summary |
Pyropia tenera (Kjellman) Kikuchi et al. (Bangiales, Rhodophyta) has been categorized to endangered species (CR+EN) in Japan. This threatened species was closely related with aqua-farming laver species, Pyropia yezoensis (Ueda) Hwang et Choi, in morphologically and phylogenetically. Firstly, we determined the distribution of P. tenera, P. yezoensis and intercross of P. tenera-yezoensis from 165 samples of first-identification as P. tenera collected at 46 localities of 17 prefectures. Sequence data of rbcL gene and ITS1 region, and PCR-RFLP (ARP4 gene) analyses elucidated that P. tenera were distributed at 38 localities, including eight new localities. Samples of intercross of P. tenera-yezoensis were mostly located in Tokai, Kanto to Tohoku regions. In SSR analysis of the pure P. tenera, we determined genotypes of three microsatellite markers (Pye13,Pye41,Pye53). Four genetic clusters (1, 2, 3 and 4) and three distinct clades (G1, G2 and G3) were detected from STRUCTURE analysis and population phylogenetic analysis, respectively. Cluster 1 was found in populations of Kumamoto and Nagasaki prefectures (G1) and highly divergent from other clusters. Cluster 2 was mainly distributed in populations along the Pacific coast from Kanto to Tohoku region (G2). Cluster 3 and 4 were detected in many populations (G3), which were geographically scattered. |
Collaborators |
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Full Text | Download (Japanese Only) |